Plaque assay and primary isolation of influenza a viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin
Identifieur interne : 002B92 ( Main/Exploration ); précédent : 002B91; suivant : 002B93Plaque assay and primary isolation of influenza a viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin
Auteurs : K. Tobita [Japon] ; A. Sugiura [Japon] ; C. Enomoto [Japon] ; M. Furuyama [Japon]Source :
- Medical Microbiology and Immunology [ 0300-8584 ] ; 1975-12-01.
English descriptors
- Teeft :
- Agar overlay medium, Amniotic cavity, Assay, Canine kidney cells, Clinical specimens, Fertile eggs, Fluid maintenance medium, Influenza, Influenza virus, Influenza viruses, Mdck, Mdck cell monolayers, Mdck cells, Parallel titration, Plaque, Plaque assay, Plaque formation, Plaquing efficiency, Primary isolation, Proteolytic, Proteolytic enzymes, Throat washings, Titration, Tobita, Trypsin, Virus, Virus strains.
Abstract
Abstract: A wide variety of influenza A viruses, comprising human, equine, porcine, and avian strains, grew productively in an established line of canine kidney cells (MDCK) under an overlay medium containing trypsin, and formed well-defined plaques regardless of their prior passage history. Plaquing efficiency was comparable to the efficiency of infection in fertile eggs via allantoic route. MDCK cells have also been successfully employed for the primary isolation of influenza A virus from throat washings of patients. Parallel titration of several clinical specimens showed that the inoculation into MDCK cells followed by incubation in the presence of trypsin was an isolation procedure as sensitive as the amniotic inoculation into fertile eggs.
Url:
DOI: 10.1007/BF02123572
Affiliations:
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Le document en format XML
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Enomoto</name><affiliation wicri:level="3"><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><wicri:orgArea>Department of Microbiology, Institute of Public Health</wicri:orgArea></affiliation><affiliation><wicri:noCountry code="subField">Okayama</wicri:noCountry></affiliation></author><author><name sortKey="Furuyama, M" sort="Furuyama, M" uniqKey="Furuyama M" first="M." last="Furuyama">M. Furuyama</name><affiliation wicri:level="3"><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><wicri:orgArea>Department of Microbiology, Institute of Public Health</wicri:orgArea></affiliation><affiliation><wicri:noCountry code="subField">Okayama</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Medical Microbiology and Immunology</title><title level="j" type="abbrev">Med Microbiol Immunol</title><idno type="ISSN">0300-8584</idno><idno type="eISSN">1432-1831</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1975-12-01">1975-12-01</date><biblScope unit="volume">162</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="9">9</biblScope><biblScope unit="page" to="14">14</biblScope></imprint><idno type="ISSN">0300-8584</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-8584</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Agar overlay medium</term><term>Amniotic cavity</term><term>Assay</term><term>Canine kidney cells</term><term>Clinical specimens</term><term>Fertile eggs</term><term>Fluid maintenance medium</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>Mdck</term><term>Mdck cell monolayers</term><term>Mdck cells</term><term>Parallel titration</term><term>Plaque</term><term>Plaque assay</term><term>Plaque formation</term><term>Plaquing efficiency</term><term>Primary isolation</term><term>Proteolytic</term><term>Proteolytic enzymes</term><term>Throat washings</term><term>Titration</term><term>Tobita</term><term>Trypsin</term><term>Virus</term><term>Virus strains</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A wide variety of influenza A viruses, comprising human, equine, porcine, and avian strains, grew productively in an established line of canine kidney cells (MDCK) under an overlay medium containing trypsin, and formed well-defined plaques regardless of their prior passage history. Plaquing efficiency was comparable to the efficiency of infection in fertile eggs via allantoic route. MDCK cells have also been successfully employed for the primary isolation of influenza A virus from throat washings of patients. Parallel titration of several clinical specimens showed that the inoculation into MDCK cells followed by incubation in the presence of trypsin was an isolation procedure as sensitive as the amniotic inoculation into fertile eggs.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Tobita, K" sort="Tobita, K" uniqKey="Tobita K" first="K." last="Tobita">K. Tobita</name></region><name sortKey="Enomoto, C" sort="Enomoto, C" uniqKey="Enomoto C" first="C." last="Enomoto">C. Enomoto</name><name sortKey="Furuyama, M" sort="Furuyama, M" uniqKey="Furuyama M" first="M." last="Furuyama">M. Furuyama</name><name sortKey="Sugiura, A" sort="Sugiura, A" uniqKey="Sugiura A" first="A." last="Sugiura">A. Sugiura</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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